Scott Westenberger, Staff FAS with Illumina, will present an introduction to the Illumina Sequencing Workflow for Next Generation Sequencing. This presentation will cover the library preparation construct needed for sequencing on Illumina platforms, and the biochemical processes of cluster generation and sequencing by synthesis that are automatically performed by Illumina sequencing devices. This will provide a general overview of the process which applies to any assay across all different sequencing instruments, providing some comparison and contrast between different chemistry versions and instrument engineering developments.