HCMNext is a multi-gene panel that can be ordered individually or on a reflex basis for patients with hypertrophic cardiomyopathy (HCM). Often, HCM can be asymptomatic and sudden death is the first and only symptom. Therefore, genetic testing may be the most effective way of identifying at-risk individuals or confirming a diagnosis. HCMNext includes 30 genes that cause HCM. These genes are implicated in over 80% of known genetic causes of HCM.

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Test Code: 8883 Test Name: HCMNext Reflex TAT: 14-21 days Genes: 30
Test Code: 8936 Test Name: HCMNext® TAT: 14-21 days Genes: 30

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We offer family variant testing at no additional cost

for all blood relatives of patients who undergo full single gene sequencing, multigene panel testing or exome sequencing at Ambry Genetics and are found to have a pathogenic or likely pathogenic variant. No-cost testing of blood relatives must be completed within 90 days of the original report date. Whenever possible, more closely related relatives should be tested before more distant relatives.

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Why Is This Important?

Knowing if your patient has a hereditary cardiovascular disorder can help you determine their future cardiovascular disease risks and guide your medical management recommendations. Key benefits include:

  1. Clarify diagnosis and risk for sudden cardiac arrest
  2. Target medical management and prevention of cardiac arrest and other complications
  3. Confirm diagnosis and identify inherited mutation following a sudden death with autopsy findings that indicate HCM
  4. Adjust management in those with HCM due to conditions like Danon and Fabry diseases
  5. Offer family members genetic testing (for a familial mutation) and implement medical surveillance to only those that need it
  6. Reduce healthcare costs, resources, and anxiety for families

When To Consider Testing

  • Patient has a family history of cardiomyopathies or a sudden unexplained death
  • Patient has a personal or family history of unexplained fainting or passing out
  • Patient has a family member with a pacemaker or implantable defibrillator (ICD) device

Mutation Distribution and Detection Rates

The HCMNext test is designed and validated to be capable of detecting >99% of described mutations in the genes represented on the tests (analytical sensitivity). The clinical sensitivity of the HCMNext test may vary widely according to the specific clinical and family history.

Test Description

HCMNext is a comprehensive analysis of 30 genes associated with HCM. Genomic deoxyribonucleic acid (gDNA) is isolated from the patient’s specimen using a standardized kit and quantified. Sequence enrichment of the targeted coding exons and adjacent intronic nucleotides is carried out by a bait-capture methodology using long biotinylated oligonucleotide probes, and is followed by polymerase chain reaction (PCR) and Next-Generation sequencing. Additional Sanger sequencing is performed for any regions missing or with insufficient read depth coverage for reliable heterozygous variant detection. Potentially homozygous variants, variants in regions complicated by pseudogene interference, and variant calls not satisfying depth of coverage and variant allele frequency quality thresholds are verified by Sanger sequencing. This assay targets all coding domains and well into the flanking 5’ and 3’ endsof all the introns and untranslated regions. Gross deletion/duplication analysis is performed for all genes using a custom pipeline based on read-depth from NGS data followed by a confirmatory orthogonal method, as needed. Exon-level resolution may not be achieved for every gene.

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