There are many choices available for genetic testing, but when it comes determining a patient’s health risks, accuracy is key. Learn how Ambry’s CancerNext provides clinicians with the most accurate results possible to better guide patient care.

When finding the answer is critical for guiding your patient’s healthcare, CancerNext is a comprehensive 34-gene panel that identifies inherited risks for at least 8 types of cancers, giving you more information to make better treatment and management decisions.

Quick Reference
Test Code 8824
Turnaround Time (TAT) 14-21 days
Number of Genes 34


Learn more about your patient’s breast or prostate cancer risk by adding AmbryScore to this test for eligible patients.

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Ordering Options

We now offer single site analysis (SSA) at no additional cost to family members

following single gene or panel testing* of the first family member (proband) within 90 days of the original Ambry report date.

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*excludes Secondary Findings and SNP Array tests

Why Is This Important?

  1. Option to modify frequency and initial age of mammogram/breast MRI, colonoscopy, prostate cancer screening, or other screening as appropriate
  2. Consideration of prophylactic mastectomy, colectomy, or other risk-reducing measures, as appropriate 
  3. Option to tailor chemotherapy strategies and/or determine eligibility for clinical trials 
  4. Identify at-risk family members 

When To Consider Testing

  • Multiple primary tumors in one person that are suspicious for a combination of hereditary breast, ovarian, colorectal, uterine cancers and/or melanoma
  • 3 or more close family members with cancers, such as breast, ovarian, colorectal, and uterine, that are suspicious for hereditary cancer 
  • Previous genetic testing was uninformative (negative or variant of uncertain significance) for a patient with a personal and/or family history suspicious for hereditary cancer

Mutation Distribution and Detection Rates*

* Excludes MUTYH carriers, and CHEK2 p.l157T

Test Description

32 genes (excluding EPCAM and GREM1) are evaluated by next generation sequencing (NGS) or Sanger sequencing of all coding domains, and well into the flanking 5’ and 3’ ends of all the introns and untranslated regions. In addition, sequencing of the promoter region is performed for the following genes: PTEN (c.-1300 to c.-745), MLH1 (c.-337 to c.-194), and MSH2 (c.-318 to c.-65). For POLD1 and POLE, missense variants located outside of the exonuclease domains (codons 311-541 and 269-485, respectively) are not routinely reported. The inversion of coding exons 1-7 of the MSH2 gene and the BRCA2 Portuguese founder mutation, c.156_157insAlu (also known as 384insAlu) are detected by NGS and confirmed by PCR and agarose gel electrophoresis.

Clinically significant intronic findings beyond 5 base pairs are always reported. Intronic variants of unknown or unlikely clinical significance are not reported beyond 5 base pairs from the splice junction. Additional Sanger sequencing is performed for any regions missing or with insufficient read depth coverage for reliable heterozygous variant detection. Reportable small insertions and deletions, potentially homozygous variants, variants in regions complicated by pseudogene interference, and single nucleotide variant calls not satisfying 100x depth of coverage and 40% het ratio thresholds are verified by Sanger sequencing.1 

Gross deletion/duplication analysis is performed for the covered exons and untranslated regions of all 34 genes using read-depth from NGS data with confirmatory multiplex ligation-dependent probe amplification (MLPA) and/or targeted chromosomal microarray. For GREM1, only the status of the 40kb 5’ UTR gross duplication is analyzed and reported. For APC, all promoter 1B gross deletions as well as single nucleotide substitutions within the promoter 1B YY1 binding motif are analyzed and reported. If a deletion is detected in exons 13, 14, or 15 of PMS2, double stranded sequencing of the appropriate exon(s) of the pseudogene, PMS2CL, will be performed to determine if the deletion is located in the PMS2 gene or pseudogene.  


1. Mu W, et al. Sanger confirmation is required to achieve optimal sensitivity and specificity in next-generation sequencing panel testing. J Mol Diagn. 2016. 18(6):923-932.

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