CONTAINER: In order of preference: 1 EDTA tube (purple top) or 2 Citric acetate tube (yellow top) or Potassium oxalate/Sodium fluoride (grey top)
AMOUNT: Adult: 3-5cc; Pediatric: 2cc minimum; Fetal/Cord blood: 2cc minimum
- Next generation sequencing panels and clinical exome sequencing require 6-10cc (adult), 5cc (pediatric) minimum
- Chromosomal microarray for newborns: 180K oligo array requires 0.5-1cc minimum; SNP Array requires 1cc minimum
- Blood samples not accepted for patients that have undergone an allogenic transplant (e.g. bone marrow or peripheral stem cell). More details listed below.*
Special Collection Notes:
- Transfusion patients: Wait at least 2 weeks after a packed cell/platelet transfusion, and at least 4 weeks after a whole blood transfusion prior to blood draw for testing.
- Chemotherapy patients: DNA quality may be affected if patient has received chemotherapy within the last 120 days. Clients will be contacted to provide additional specimen if DNA quality is insufficient.
STORAGE: Room temperature at 15-30 °C (short-term). Refrigerated at 2-8 °C (long-term). DO NOT FREEZE.
STABILITY: When stored per above, stable for 7 days, refrigerated. Stability may be affected if stored at room temperature for more than 3 days. Please contact Ambry if your sample is more than 7 days old.
SHIPPING: For whole blood, ship ambient for 2-day delivery (preferred) using the Ambry Sample Submission Kit, or similar. For fetal/cord blood, please call ahead and ship ambient for next-day delivery (required). Please do not ship to arrive on a holiday.
*Please contact Ambry at +1 949-900-5500 before sending fetal/cord blood specimens. Maternal cell contamination (MCC) analysis required for all fetal/cord blood tests. Please send a maternal sample, using a separate requisition. An additional charge applies. We recommend sending parental blood samples for mutation confirmation, if previous parental testing was done elsewhere. Chromosomal microarray analysis is not available on prenatal samples.
**Patients with a history of active/recent hematological disease, (e.g. leukemia, lymphoma, or myelodysplastic syndrome) will be subject to additional review prior to accessioning. Blood specimens in patients with hematological disease may not be accepted in some cases. For these cases and those who have undergone allogenic transplant (e.g. bone marrow or peripheral stem cell transplant), cultured fibroblasts are preferred, though fresh/fresh frozen normal tissue (no fixatives) may be accepted in some cases. Please refer to “Cultured Cell” section (see tab above) for those specimen requirements. If you are sending fresh/fresh frozen normal tissue samples, please call Ambry to discuss before sample submission.