Digital PCR

Digital PCR (dPCR) partitions a sample into hundreds of individual real-time reactions, increasing sensitivity and enabling researchers to accurately quantitate allele copy number.

dPCR has a wide range of applications, including detecting low-fold copy number variations, rare allele detection, haplotyping and gene expression analysis.  Ambry Genetics offers reliable dPCR results utilizing the integrated fluidic circuits (IFCs) on the Fluidigm Digital Array™.

Features
  • Up to 12 samples can be processed in three hours
  • Simple, fast and reliable workflow
  • Precise and reproducible measurements of target copy number
Applications
  • Quantification of nucleic acid standards
  • Quantification of next-gen sequencing libraries
  • Rare allele detection
  • Low fold copy number discrimination
  • Enrichment and separation of mixtures
  • High-resolution CNV
  • Gene copy number
Platforms 
Fluidigm BioMark™ HD System